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Code Name Applications Availability Size Tested species Price Grade  
EB08657 Goat Anti-USP16 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
Click here to download PDF datasheet.

Target Protein

Principal Names: USP16, ubiquitin specific peptidase 16, UBP-M, deubiquitinating enzyme 16, ubiquitin carboxyl-terminal hydrolase 16, ubiquitin specific protease 16, ubiquitin thiolesterase 16, ubiquitin-specific processing protease 16
Official Symbol: USP16
Accession Number(s): NP_001027582.1; NP_001001992.1
Human GeneID(s): 10600
Non-Human GeneID(s): 74112 (mouse), 288306 (rat)
Important Comments: Reported variants represent identical protein: NP_001027582.1 NP_006438.1

Immunogen

Peptide with sequence NVEWNICQDCKTDNK, from the internal region of the protein sequence according to NP_001027582.1; NP_001001992.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Horse, Dog, Cow

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