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Goat Anti-SUR1 / ABCC8 Antibody (EB08736)

Related Products: EBP08736 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08736 Goat Anti-SUR1 / ABCC8 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08736 1
EB08736 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABCC8, SUR1, ATP-binding cassette, sub-family C (CFTR/MRP), member 8, ABC36, HHF1, HI, HRINS, MRP8, PHHI, SUR, TNDM2, ATP-binding cassette, sub-family C, member 8, sulfonylurea receptor (hyperinsulinemia)
Official Symbol: ABCC8
Accession Number(s): NP_000343.2
Human GeneID(s): 6833
Non-Human GeneID(s): 20927 (mouse), 25559 (rat)

Immunogen

Peptide with sequence C-EFDKPEKLLSRKD, from the C Terminus of the protein sequence according to NP_000343.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 177kDa according to NP_000343.2). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human; Mouse, Rat, Dog

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