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Goat Anti-SIGLEC8 Antibody (EB09019)

Related Products: EBP09019 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09019 Goat Anti-SIGLEC8 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09019 1
EB09019 (0.3µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SIGLEC8, sialic acid binding Ig-like lectin 8, MGC59785, SAF2, SIGLEC-8, SIGLEC8L
Official Symbol: SIGLEC8
Accession Number(s): NP_055257.2
Human GeneID(s): 27181

Immunogen

Peptide with sequence C-SYKSQLNYKTKQ, from the internal region of the protein sequence according to NP_055257.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 Jun 15;101(12):5014-20.; PMID: 12609831). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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