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Goat Anti-BTLA Antibody (EB09035)

Related Products: EBP09035 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09035 Goat Anti-BTLA Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09035 1
EB09035 (1µg/ml) staining of Mouse Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BTLA, B and T lymphocyte associated, BTLA1, CD272, FLJ16065, MGC129743, B and T lymphocyte attenuator
Official Symbol: BTLA
Accession Number(s): NP_861445.3; NP_001078826.1
Human GeneID(s): 151888
Non-Human GeneID(s): 208154 (mouse), 407756 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_861445.3; NP_001078826.1).

Immunogen

Peptide with sequence KEAPTEYASICVRS, from the C Terminus of the protein sequence according to NP_861445.3; NP_001078826.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat

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