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Goat Anti-ZO-1 Antibody (EB09206)

Related Products: EBP09206 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09206 Goat Anti-ZO-1 Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09206 1
EB09206 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09206 2
EB09206 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue) Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TJP1, tight junction protein 1 (zona occludens 1), DKFZp686M05161, MGC133289, ZO-1, OTTHUMP00000174520, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
Official Symbol: TJP1
Accession Number(s): NP_003248.3; NP_783297.2
Human GeneID(s): 7082
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)

Immunogen

Peptide with sequence C-PKPTSQNQFSEHDKT, from the internal region of the protein sequence according to NP_003248.3; NP_783297.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in the cellular junction of A431 cells, and in the plasma membrane and cytoplasm of U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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