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Goat Anti-XPNPEP1 Antibody (EB09415)

Related Products: EBP09415 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09415 Goat Anti-XPNPEP1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09415 1
EB09415 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09415 2
EB09415 (0.1µg/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09415 3
EB09415 (0.1µg/ml) staining of Pig Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09415 4
EB09415 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: XPNPEP1, X-prolyl aminopeptidase (aminopeptidase P) 1, soluble, SAMP, XPNPEP, XPNPEPL, XPNPEPL1, OTTHUMP00000020457, OTTHUMP00000058856, X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (SAMP, XPNPEP, XPNPEPL), X-prolyl aminopeptidase (aminopeptidase P)-like
Official Symbol: XPNPEP1
Accession Number(s): NP_065116.2; NP_001161076.1
Human GeneID(s): 7511
Non-Human GeneID(s): 170750 (mouse), 170751 (rat)
Important Comments: This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).

Immunogen

Peptide with sequence C-LIRETQPISKQH, from the C Terminus of the protein sequence according to NP_065116.2; NP_001161076.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines, and 75kDa band observed in Pig Pancreas lysate (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig, Dog

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