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Goat Anti-TLR4 / CD284 Antibody (EB09441)

Related Products: EBP09441 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09441 Goat Anti-TLR4 / CD284 Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09441 1
EB09441 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/ER and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09441 2
EB09441 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09441 3
EB09441 (6µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09441 4
EB09441 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
EB09441 5
EB09441 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TLR4, toll-like receptor 4, ARMD10, CD284, TOLL, hToll, OTTHUMP00000022807, homolog of Drosophila toll
Official Symbol: TLR4
Accession Number(s): NP_612564.1
Human GeneID(s): 7099
Non-Human GeneID(s): 21898 (mouse), 29260 (rat)

Immunogen

Peptide with sequence C-YSSQDEDWVRNE, from the Internal region of the protein sequence according to NP_612564.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells and membrane and cytoplasm of THP-1 cells. Recommended concentration: 10µg/ml

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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