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Goat Anti-eIF5A Antibody (EB09590)

Related Products: EBP09590 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09590 Goat Anti-eIF5A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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Fax. (888) 832-2613
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EB09590 1
EB09590 (0.1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09590 2
EB09590 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09590 3
EB09590 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09590 4
EB09590 (3.75µg/ml) staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EIF5A, eukaryotic translation initiation factor 5A, EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI, OTTHUMP00000128384, uORF A
Official Symbol: EIF5A
Accession Number(s): NP_001137232.1
Human GeneID(s): 1984
Important Comments: Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:

Immunogen

Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of the protein sequence according to NP_001137232.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 20kDa band observed in MOLT4 lysates (calculated MW of 20.2kDa according to NP_001137232.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 27414022 and 33459432.
IHC: Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in Western blot on Human:
Karina D Pereira, Letícia Tamborlin, Tanes I de Lima, Silvio R Consonni, Leonardo R Silveira, Augusto D Luchessi
Alternative human eIF5A protein isoform plays a critical role in mitochondria
J Cell Biochem. 2021 Jan 18.
PMID: 33459432

This antibody has been successfully used in Western blot on Human:
Pereira KD, Tamborlin L, Meneguello L, de Proença AR, Almeida IC, Lourenço RF, Luchessi AD.
Alternative Start Codon Connects eIF5A to Mitochondria.
J Cell Physiol. 2016 Dec;231(12):2682-9.
PMID: 27414022

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