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Goat Anti-TET2 Antibody (EB09642)

Related Products: EBP09642 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09642 Goat Anti-TET2 Antibody Pep-ELISA, IHC, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB09642 1
EB09642 (4µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09642 2
EB09642 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TET2, tet oncogene family member 2, FLJ20032, KIAA1546, MGC125715, OTTHUMP00000161869
Official Symbol: TET2
Accession Number(s): NP_001120680.1; NP_060098.3
Human GeneID(s): 54790
Non-Human GeneID(s): 214133 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001120680.1; NP_060098.3).

Immunogen

Peptide with sequence C-PHPQSNNDQQREGSF, from the internal region of the protein sequence according to NP_001120680.1; NP_060098.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: In paraffin embedded Human Spleen shows nuclear staining in select splenocytes. Recommended concentration: 2-6µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

Specific References

This antibody (previous batch) has been successfully used in IHC on Mouse:
Rakoczy J, Padmanabhan N, Krzak AM, Kieckbusch J, Cindrova-Davies T, Watson ED
Dynamic expression of TET1, TET2, and TET3 dioxygenases in mouse and human placentasthroughout gestation.
Placenta. 2017 Nov;59:46-56.
PMID: 29108636

This antibody (previous batch) has been successfully used in IHC on Human:
Orr BA, Haffner MC, Nelson WG, Yegnasubramanian S, Eberhart CG.
Decreased 5-hydroxymethylcytosine is associated with neural progenitor phenotype in normal brain and shorter survival in malignant glioma.
PLoS One. 2012;7(7):e41036. 2012 Jul 19
PMID: 22829908

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