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Goat Anti-Mesothelin Antibody (EB09808)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB09808 | Goat Anti-Mesothelin Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB09808 (0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: CAK1, megakaryocyte potentiating factor, mesothelin, MPF, SMR, MSLNOfficial Symbol: MSLN
Accession Number(s): NP_005814.2; NP_037536.2
Human GeneID(s): 10232
Important Comments: This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
Immunogen
Peptide with sequence CPSGKKAREIDES, from the internal region of the protein sequence according to NP_005814.2; NP_037536.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 68.1kDa according to NP_005814.2 and of 69.0kDa according to NP_037536.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species Reactivity
Tested: HumanExpected from sequence similarity: Human