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Goat Anti-BICC1 (aa223-234) Antibody (EB09818)

Related Products: EBP09817 - Immunizing Peptide, EBP09818 - Immunizing Peptide, Goat Anti-BICC1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09818 Goat Anti-BICC1 (aa223-234) Antibody Pep-ELISA, IP Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09818 1
HEK293 overexpressing Human BICC1 with N-terminal FLAG, probed with rabbit anti-BCC1 in Western Blot after IP using either EB09818 or anti-FLAG antibody in the presence or absence of SDS.
Click here to download PDF datasheet.

Target Protein

Principal Names: bicaudal C homolog 1, bicaudal C homolog 1 (Drosophila), DKFZp686I21119, BICC1
Official Symbol: BICC1
Accession Number(s): NP_001073981.1
Human GeneID(s): 80114
Non-Human GeneID(s): 361832 (rat)

Immunogen

Peptide with sequence C-PNSPSIQHISQT, from the internal region of the protein sequence according to NP_001073981.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments in Human Brain (Cerebellum), Kidney, Lung and Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Immunoprecipitation:Immunoprecipitation by EB09818 from lysates of HEK293 transfected and overexpressing FLAG-HA-BICC1. Precipitation works best in the presence of SDS. Data kindly provided by anonymous customer.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Dog, Cow

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