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Goat Anti-SPAG16 Antibody (EB09825)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB09825 | Goat Anti-SPAG16 Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB09825 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, sperm associated antigen 16, sperm-associated WD repeat protein, WD repeat domain 29, WDR29, SPAG16Official Symbol: SPAG16
Accession Number(s): NP_078808.3
Human GeneID(s): 79582
Non-Human GeneID(s): 66722 (mouse)
Important Comments: This antibody is expected to recognize isoform 1 (NP_078808.3) only.
Immunogen
Peptide with sequence C-KLHYASYEPTIR, from the internal region of the protein sequence according to NP_078808.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave bands at approx 55kDa and 22kDa in Human Testis lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 70.8kDa according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Dog, Cow