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Goat Anti-CHCHD3 (aa151-164) Antibody (EB09840)

Related Products: EBP09839 - Immunizing Peptide, EBP09840 - Immunizing Peptide, Goat Anti-CHCHD3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09840 Goat Anti-CHCHD3 (aa151-164) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB09840 1
EB09840 (0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09840 2
EB09840 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420, CHCHD3
Official Symbol: CHCHD3
Accession Number(s): NP_060282.1
Human GeneID(s): 54927
Non-Human GeneID(s): 66075 (mouse), 296966 (rat)

Immunogen

Peptide with sequence C-RSSEFYRVTTEQYQ, from the internal region of the protein sequence according to NP_060282.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26.2kDa band observed in Human Placenta lysates (calculated MW of 26kDa according to NP_060282.1). Recommended concentration: 0.3-1µg/ml. A customer reported ~30kDa in THP1 and in human primary monocyte derived dendritic cells.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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