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Goat Anti-AIM2 Antibody (EB09874)

Related Products: EBP09873 - Immunizing Peptide, EBP09874 - Immunizing Peptide, Goat Anti-AIM2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09874 Goat Anti-AIM2 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09874 1
EB09874 (0.75µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09874 2
EB09874 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09874 3
EB09874 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09874 4
EB09874 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09874 5
EB09874 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: absent in melanoma 2, PYHIN4, AIM2
Official Symbol: AIM2
Accession Number(s): NP_004824.1; NP_001335176.1
Human GeneID(s): 9447

Immunogen

Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1; NP_001335176.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 37kDa band observed in lysates of cell line HeLa (calculated MW of 39.0kDa according to Human NP_004824.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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