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Goat Anti-Spondin 2 / Mindin Antibody (EB09965)

Code Name Applications Availability Size Tested species Price Grade  
EB09965 Goat Anti-Spondin 2 / Mindin Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $255.00 Aspiring

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Target Protein

Principal Names: DIL1, DIL-1, Mindin, M-spondin, spondin 2, extracellular matrix protein, SPON2
Official Symbol: SPON2
Accession Number(s): NP_036577.1
Human GeneID(s): 10417
Important Comments: Reported variants NP_036577.1 and NP_001121797.1 represent identical protein.

Immunogen

Peptide with sequence C-RKNQYVSNGLRDFA, from the internal region of the protein sequence according to NP_036577.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 35kDa band observed in preliminary testing of Human and Mouse Prostate lysate after 1µg/ml antibody staining (calculated MW of 35.8kDa according to Human NP_036577.1). Primary incubation 1 hour at room temperature.

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Cow