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Goat Anti-POU6F2 Antibody (EB10042)

Related Products: EBP10042 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10042 Goat Anti-POU6F2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10042 1
EB10042 (0.3µg/ml) staining of Mouse Eye lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: POU class 6 homeobox 2, POU domain, class 6, transcription factor 2, retina-derived POU-domain factor-1, RPF-1, Wilms tumor suppressor locus, WT5, WTSL, POU6F2
Official Symbol: POU6F2
Accession Number(s): NP_009183.3; NP_001159490.1
Human GeneID(s): 11281
Important Comments: This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).

Immunogen

Peptide with sequence RSEMNAELRGEDK-C, from the N Terminus of the protein sequence according to NP_009183.3; NP_001159490.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 65kDa band observed in Mouse Eye lysates (calculated MW of 73.3kDa according to Human NP_009183.3 and Mouse NP_778171.2). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow

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