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Goat Anti-NIPBL (aa889-902) Antibody (EB10055)

Related Products: EBP10055 - Immunizing Peptide, EBP10056 - Immunizing Peptide, Goat Anti-NIPBL (aa922-935) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10055 Goat Anti-NIPBL (aa889-902) Antibody Pep-ELISA, IHC, IF Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB10055 1
EB10055 (2µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB10055 2
EB10055 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
EB10055 3
EB10055 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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Target Protein

Principal Names: CDLS, CDLS1, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3, IDN3-B, Nipped-B homolog (Drosophila), nipped-B-like, Scc2, SCC2 homolog, sister chromatid cohesion 2 homolog, NIPBL
Official Symbol: NIPBL
Accession Number(s): NP_597677.2; NP_056199.2
Human GeneID(s): 25836
Non-Human GeneID(s): 71175 (mouse), 294787 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).

Immunogen

Peptide with sequence C-RPDSPRVKQGDSNK, from the internal region of the protein sequence according to NP_597677.2; NP_056199.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 2µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow