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Goat Anti-ZDHHC1 (aa213-222) Antibody (EB10422)

Code Name Applications Availability Size Tested species Price Grade  
EB10422 Goat Anti-ZDHHC1 (aa213-222) Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

Ordering Information for

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

Target Protein

Principal Names: C16orf1, DHHC-domain-containing cysteine-rich protein, HSU90653, zinc finger, DHHC domain containing 1, zinc finger, DHHC-type containing 1, ZNF377, ZDHHC1
Official Symbol: ZDHHC1
Accession Number(s): NP_037436.1
Human GeneID(s): 29800
Non-Human GeneID(s): 70796 (mouse), 291967 (rat)

Immunogen

Peptide with sequence C-TNRHFEVLKN, from the internal region of the protein sequence according to NP_037436.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35kDa band in Human Heart, Liver and Uterus lysates after 0.5 µg/ml antibody staining and also in fetal Mouse Heart, Kidney ans Lung lysates after 0.1ug/ml antibody staining . Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 54.8kDa according to NP_037436.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat

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