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Goat Anti-SEPT4 (aa425-435) Antibody (EB10427)

Related Products: EBP10427 - Immunizing Peptide, EBP10428 - Immunizing Peptide, Goat Anti-SEPT4 (aa33-44) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10427 Goat Anti-SEPT4 (aa425-435) Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10427 1
EB10427 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10427 2
EB10427 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10427 3
EB10427 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARTS, BRADEION, CE5B3, cell division control-related protein 2, cerebral protein 7, H5, hCDCREL-2, hucep-7, MART, peanut-like 2, PNUTL2, SEP4, septin 4
Official Symbol: SEPT4
Accession Number(s): NP_004565.1; NP_536341.1, NP_001185642.1
Human GeneID(s): 5414
Non-Human GeneID(s): 18952 (mouse)
Important Comments: This antibody is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.

Immunogen

Peptide with sequence C-RNKLTRESGTD, from the internal region of the protein sequence according to NP_004565.1; NP_536341.1, NP_001185642.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 50kDa band observed in Human Heart lysates and in preliminary testing of Mouse Brain lysate (calculated MW of 53.0kDa according to NP_536341.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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