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Goat Anti-CYP24A1 Antibody (EB10490)

Related Products: EBP10490 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10490 Goat Anti-CYP24A1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10490 1
EB10490 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10490 2
EB10490 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 1,25-@dihydroxyvitamin D3 24-hydroxylase, 24-OHase, CP24, CYP24, cytochrome P450 family 24 subfamily A polypeptide 1, cytochrome P450, family 24, subfamily A, polypeptide 1, cytochrome P450, subfamily XXIV (vitami, MGC126273, MGC126274, P450-CC24, CYP24A1
Official Symbol: CYP24A1
Accession Number(s): NP_000773.2; NP_001122387.1
Human GeneID(s): 1591
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000773.2; NP_001122387.1).

Immunogen

Peptide with sequence C-RKYDIQATDN, from the internal region of the protein sequence according to NP_000773.2; NP_001122387.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in Human and Rat Liver lysates (calculated MW of 51.2kDa according to NP_001122387.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skin. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

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