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Goat Anti-SIM2 Antibody (EB10569)

Related Products: EBP10569 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10569 Goat Anti-SIM2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10569 1
EB10569 (0.5µg/ml) staining of fetal Mouse kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SIM2, single-minded homolog 2 (Drosophila), MGC119447, SIM, bHLHe15, OTTHUMP00000109044, class E basic helix-loop-helix protein 15, single-minded homolog 2, single-minded homolog 2, short isoform
Official Symbol: SIM2
Accession Number(s): NP_005060.1; NP_033664.2
Human GeneID(s): 6493
Non-Human GeneID(s): 20465 (mouse), 304071 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_005060.1; NP_033664.2).

Immunogen

Peptide with sequence C-SDLLYTPSYS, from the internal region of the protein sequence according to NP_005060.1; NP_033664.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 80kDa band observed in fetal Mouse Kidney lysates (calculated MW of 73.2kDa according to Human NP_005060.1) and 72.5kDa according to Mouse NP_035507.2. Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat

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