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Goat Anti-Mucin 19 / Apomucin Antibody (EB10619)

Related Products: EBP10619 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10619 Goat Anti-Mucin 19 / Apomucin Antibody Pep-ELISA, WB, ICC/ IF Yes 100µg specific antibody in 200µl Mouse $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10619 1
EB10619 (0.25 µg/ml) staining of homogenized Mouse salivary gland tissue. (20 µg protein per lane). Detected by chemiluminescence.
EB10619 2
EB10619 (1.8ug/ml) staining cells of the sublingual salivary gland (SLG) in mouse, but not any cells of the submandibular salivary gland (SMG).
Click here to download PDF datasheet.

Target Protein

Principal Names: Muc19, mucin 19, MUC-19, gel-forming secreted mucin-19, mucin-19, sublingual apomucin, mucin apoprotein
Official Symbol: Muc19
Accession Number(s): NP_997126.2
Non-Human GeneID(s): 239611 (mouse), 497227 (rat)

Immunogen

Peptide with sequence ECKRSVKYNYETFQ, from the C Terminus of the protein sequence according to NP_997126.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: A customer observed high MWt bands in salivary gland tissue from female or male C57BL/6 mice: Proteins were separated by electrophoresis through 3-8% gradient gels, transferred to nitrocellulose, and probed with primary antibody at 1:2000 dilution (0.25 µg/ml), followed by peroxidase-conjugated anti-goat at 1:50,000.

ICC/ IF: Positive staining in the sublingual salivary gland of the mouse, while cells remain negative in the submandibular salivary gland. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse, Rat

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