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Goat Anti-CD28 Antibody (EB10740)

Related Products: EBP10740 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10740 Goat Anti-CD28 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10740 1
EB10740 (0.3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10740 2
EB010740 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB10740 3
EB10740 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD28, CD28 molecule, MGC138290, Tp44, CD28 antigen, CD28 antigen (Tp44), T-cell-specific surface glycoprotein
Official Symbol: CD28
Accession Number(s): NP_006130.1
Human GeneID(s): 940
Important Comments: The immunizing peptide corresponds to a part of the extracellular domain.

Immunogen

Peptide with sequence SQQLQVYSKTGFNCD, from the internal region of the protein sequence according to NP_006130.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 48kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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