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Goat Anti-ZEB2 (aa545-558) Antibody (EB10813)

Related Products: EBP10813 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10813 Goat Anti-ZEB2 (aa545-558) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10813 1
EB10813 (0.2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10813 2
EB10813 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ZEB2, zinc finger E-box binding homeobox 2, FLJ42816, KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B, OTTHUMP00000204540, Smad-interacting protein 1, zinc finger E-box-binding homeobox 2, zinc finger homeobox 1b, zinc finger homeobox protein 1
Official Symbol: ZEB2
Accession Number(s): NP_055610.1; NP_001165124.1
Human GeneID(s): 9839
Non-Human GeneID(s): 24136 (mouse), 311071 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_055610.1; NP_001165124.1).

Immunogen

Peptide with sequence C-TDSRRQISNIKKEK, from the internal region of the protein sequence according to NP_055610.1; NP_001165124.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100+38kDa bands observed in Human Liver lysates (calculated MW of 136kDa according to NP_055610.1). Recommended concentration: 0.2-0.5µg/ml. Primary incubation was 1 hour. We believe the two observed bands represent two breakdown fragments.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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