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Goat Anti-Hsd11b1 (mouse) Antibody (EB11062)

Related Products: EBP11062 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11062 Goat Anti-Hsd11b1 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11062 1
EB11062 (0.3µg/ml) staining of Mouse Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11062 2
EB11062 (0.3µg/ml) staining of Mouse Lung lysate (60µg protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence with Li-cor). Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK
Click here to download PDF datasheet.

Target Protein

Principal Names: 11-beta-HSD1, 11-beta-hydroxysteroid dehydrogenase 1, 11-DH, corticosteroid 11-beta-dehydrogenase isozyme 1, HDL, HSD11, HSD11B, HSD11L, hydroxysteroid (11-beta) dehydrogenase 1, short chain dehydrogenase/reductase family 26C, member 1, Hsd11b1
Official Symbol: Hsd11b1
Accession Number(s): NP_032314.2
Non-Human GeneID(s): 15483 (mouse), 25116 (rat)

Immunogen

Peptide with sequence C-TARSEEGLQKVVSR, from the internal region of the protein sequence according to NP_032314.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse, Rat

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