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Code Name Applications Availability Size Tested species Price Grade  
EB11081 Goat Anti-Transglutaminase 2 (aa578-591) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11081 1
EB11081 (2µg/ml) staining of Human Placenta (A) and (3ug/ml) Mouse Kidney (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: C polypeptide, G-ALPHA-h, GNAH, protein-glutamine gamma-glutamyltransferase 2, protein-glutamine-gamma-glutamyltransferase, TG(C), TG2, TGase C, TGase H, TGase-2, TGase-H, TGC, tissue transglutaminase, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), transglutaminase C, transglutaminase H, transglutaminase-2, TGM2
Official Symbol: TGM2
Accession Number(s): NP_004604.2; NP_001310245.1; NP_001310247.1; NP_001310246.1
Human GeneID(s): 7052
Non-Human GeneID(s): 21817 (mouse), 56083 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms.

Immunogen

Peptide with sequence C-ERDLYLENPEIKIR, from the internal region of the protein sequence according to NP_004604.2; NP_001310245.1; NP_001310247.1; NP_001310246.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 80kDa band observed in Human Placenta and Mouse Kidney lysates, and in preliminary testing of Mouse Heart and Lung lysate (calculated MW of 77.3kDa according to Human NP_004604.2 and 77.1kDa according to Mouse NP_033399.1 ). Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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