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Code Name Applications Availability Size Tested species Price Grade  
EB11097 Goat Anti-UNC5B (aa629-643) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11097 1
EB11097 (2µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: netrin receptor UNC5B, p53RDL1, p53-regulated receptor for death and life protein 1, protein unc-5 homolog 2, protein unc-5 homolog B, transmembrane receptor Unc5H2, unc-5 homolog 2, unc-5 homolog B (C. elegans), UNC5H2, UNC5B
Official Symbol: UNC5B
Accession Number(s): NP_734465.2
Human GeneID(s): 219699
Non-Human GeneID(s): 107449 (mouse), 60630 (rat)

Immunogen

Peptide with sequence C-SARDWIFQLKTQAHQ, from the internal region of the protein sequence according to NP_734465.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 90kDa band observed in Human Bone Marrow lysates (calculated MW of 104kDa according to NP_734465.2). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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