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Goat Anti-CDH1 (aa662-675) Antibody (EB11206)

Related Products: EBP11206 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11206 Goat Anti-CDH1 (aa662-675) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11206 1
EB11206 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11206 2
EB11206 (0.3µg/ml) staining of Pig Kidney (A) and Colon (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11206 3
EB11206 (0.3µg/ml) staining of Mouse Lung (A) and Rat Lung (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11206 4
EB11206 (5µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CDHE, cell-CAM 120/80, ECAD, E-Cadherin, epithelial cadherin, LCAM, UVO, uvomorulin, CDH1
Official Symbol: CDH1
Accession Number(s): NP_004351.1
Human GeneID(s): 999
Non-Human GeneID(s): 83502 (rat)
Important Comments: The immunizing peptide represents part of the extracellular domain.

Immunogen

Peptide with sequence C-DYKINLKLMDNQNK, from the internal region of the protein sequence according to NP_004351.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 85kDa band observed in Human Kidney lysates and in Pig Colon, Kidney and Lung lysates and ~90kDa in Mouse and Rat Lung lysates (calculated MW of 97.5kDa according to NP_004351.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Thyroid. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Rat, Dog, Pig

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