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Goat Anti-TRIM11 (aa249-263) Antibody (EB11287)

Code Name Applications Availability Size Tested species Price Grade  
EB11287 Goat Anti-TRIM11 (aa249-263) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB11287 1
EB11287 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11287 2
EB11287 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BIA1, E3 ubiquitin-protein ligase TRIM11, RING finger protein 92, RNF92, tripartite motif containing 11, tripartite motif-containing 11, tripartite motif-containing protein 11, TRIM11
Official Symbol: TRIM11
Accession Number(s): NP_660215.1
Human GeneID(s): 81559

Immunogen

Peptide with sequence C-DALRRVQDVKLQPPE, from the internal region of the protein sequence according to NP_660215.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 52kDa band observed in Human Spleen lysates (calculated MW of 52.8kDa according to NP_660215.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Cow

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