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Goat Anti-ARPC1A Antibody (EB11355)

Related Products: EBP05434 - Immunizing Peptide, EBP11355 - Immunizing Peptide, Goat Anti-ARPC1A Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11355 Goat Anti-ARPC1A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11355 1
EB11355 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11355 2
EB11355 (0.1µg/ml) staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11355 3
EB11355 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: actin binding protein (Schizosaccharomyces pombe sop2-like), actin related protein 2/3 complex, subunit 1A, 41kDa, actin-related protein 2/3 complex subunit 1A, Arc40, OTTHUMP00000206034, SOP2Hs, SOP2L, SOP2-like protein, ARPC1A
Official Symbol: ARPC1A
Accession Number(s): NP_006400.2; NP_001177925.1
Human GeneID(s): 10552
Non-Human GeneID(s): 56443 (mouse), 81824 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B.

Immunogen

Peptide with sequence C-DFKTLESSIQGLR, from the C Terminus of the protein sequence according to NP_006400.2; NP_001177925.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 45kDa band observed in lysates of cell line HeLa (calculated MW of 41.6kDa according to NP_006400.2). Approx 37kDa band observed in fetal Mouse Brain and adult Rat Brain lysates (calculated MW of 41.6kDa according to Mouse NP_062741.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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