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Code Name Applications Availability Size Tested species Price Grade  
EB11392 Goat Anti-STAT5A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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EB11392 1
EB11392 (0.5µg/ml) staining of K562 (A) and (0.3µg/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
EB11392 2
EB11392 (0.5µg/ml) staining of Mouse Lymph node lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
EB11392 3
EB11392 (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11392 4
EB11392 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGF, signal transducer and activator of transcription 5A, STAT5, STAT5A
Official Symbol: STAT5A
Accession Number(s): NP_003143.2
Human GeneID(s): 6776
Non-Human GeneID(s): 20850 (mouse), 24918 (rat)
Important Comments: This antibody is not expected to cross-react with STAT5B.

Immunogen

Peptide with sequence C-DSRLSPPAGLFTSAR, from the C Terminus of the protein sequence according to NP_003143.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 100kDa band observed in lysates of cell line NIH3T3 and in Mouse Lymph node lysates and approx. 90-100kDa in lysates of cell line K562 (calculated MW of 90.6kDa according to Human NP_003143.2 and 90.8kDa according to Mouse NP_001349609.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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