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Goat Anti-TBP /Transcription factor IID (aa39-50) Antibody (EB11402)

Related Products: EBP11401 - Immunizing Peptide, EBP11402 - Immunizing Peptide, Goat Anti-TBP /Transcription factor IID (isoform1) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11402 Goat Anti-TBP /Transcription factor IID (aa39-50) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc
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EB11402 1
EB11402 (1µg/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11402 2
EB11402 (1µg/ml) staining of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
EB11402 3
EB11402 (1µg/ml) staining of Pig Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TATA box binding protein, TATA sequence-binding protein, TATA-box binding protein N-terminal domain, TATA-box factor, TATA-box-binding protein, TFIID, transcription initiation factor TFIID TBP subunit, TBP
Official Symbol: TBP
Accession Number(s): NP_003185.1; NP_001165556.1
Human GeneID(s): 6908
Non-Human GeneID(s): 21374 (mouse), 117526 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003185.1; NP_001165556.1).

Immunogen

Peptide with sequence C-TPQPIQNTNSLS, from the internal region of the protein sequence according to NP_003185.1; NP_001165556.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 40kDa band observed in nuclear, but not in cytosolic lysates of cell line HeLa (calculated MW of 37.7kDa according to NP_003185.1) and 37kDa observed in lysates of NIH3T3 and of Mouse and Rat Testis lysates (calculated MW of 34.7kDa according to Mouse NP_038712.3 and of 35.0kDa according to Rat NP_001004198.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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