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Goat Anti-ZBTB24 (aa232-245) Antibody (EB11414)

Related Products: EBP11414 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11414 Goat Anti-ZBTB24 (aa232-245) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11414 1
EB11414 (1µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11414 2
EB11414 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BIF1, ICF2, OTTHUMP00000016968, PATZ2, POZ (BTB) and AT hook containing zinc finger 2, zinc finger and BTB domain containing 24, zinc finger and BTB domain-containing protein 24, zinc finger protein 450, ZNF450, ZBTB24
Official Symbol: ZBTB24
Accession Number(s): NP_055612.2; NP_001157785.1
Human GeneID(s): 9841
Important Comments: This antibody is expected to recognize both reported isoforms (NP_055612.2; NP_001157785.1).

Immunogen

Peptide with sequence C-PVEKDENYDPKTED, from the internal region of the protein sequence according to NP_055612.2; NP_001157785.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 80kDa band observed in lysates of cell line Daudi (calculated MW of 78.3kDa according to NP_055612.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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