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Goat Anti-DYRK1A Antibody (EB11483)

Related Products: EBP11483 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11483 Goat Anti-DYRK1A Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Waltham, MA 02452
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Email: [email protected]
www.arp1.com
EB11483 1
EB11483 (0.5µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: dual specificity tyrosine-phosphorylation-regulated kinase 1A, dual specificity YAK1-related kinase, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, DYRK, DYRK1, DYRK1A, hMNB, HP86, MNB, mnb protein kinase homolog hp86, MNB/DYRK protein kinase, MNBH, MRD7, OTTHUMP00000109090, OTTHUMP00000109091, OTTHUMP00000109094, OTTHUMP00000174799, protein kinase minibrain homolog, serine/threonine kinase MNB, serine/threonine-specific protein kinase
Official Symbol: DYRK1A
Accession Number(s): NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1
Human GeneID(s): 1859
Non-Human GeneID(s): 13548 (mouse), 25255 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1). This antibody is not expected to cross-react with DYRK1B.

Immunogen

Peptide with sequence PHSHQYSDRRQPN-C, from the N Terminus of the protein sequence according to NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 80kDa band observed in lysates of cell line K562 (calculated MW of 85.6kDa according to NP_001387.2). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.

Immunoprecipitation: This product has been successfully used in IP on Human: Widowati et al, BMC Res Notes. 2018 May 15;11(1):297. PMID: 29764512.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in IP on Human:
Esti Wahyu Widowati, Simone Bamberg-Lemper and Walter Becky
Mutational analysis of two residues in the DYRK homology box of the protein kinase DYRK1A
BMC Res Notes. 2018 May 15;11(1):297. 
PMID: 29764512

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