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Goat Anti-PRMT7 Antibody (EB11563)

Related Products: EBP11563 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11563 Goat Anti-PRMT7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB11563 1
EB11563 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: [Myelin basic protein]-arginine N-methyltransferase PRMT7, FLJ10640, histone-arginine N-methyltransferase PRMT7, KIAA1933, myelin basic protein-arginine N-methyltransferase, OTTHUMP00000174863, PRMT7, protein arginine methyltransferase 7, protein arginine N-methyltransferase 7
Official Symbol: PRMT7
Accession Number(s): NP_061896.1; NP_001171753.1
Human GeneID(s): 54496
Non-Human GeneID(s): 214572 (mouse), 361402 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_061896.1; NP_001171753.1).

Immunogen

Peptide with sequence C-PRFGEINDQDRTDR, from the internal region of the protein sequence according to NP_061896.1; NP_001171753.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75kDa band observed in lysates of cell line HeLa and of Mouse Spleen (calculated MW of 78.5kDa according to Human NP_061896.1 and 78.3kDa according to Mouse NP_663379.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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