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Goat Anti-Fubp1 (mouse, aa160-174) Antibody (EB11620)

Related Products: EBP11620 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11620 Goat Anti-Fubp1 (mouse, aa160-174) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB11620 1
EB11620 (0.03µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11620 2
EB11620 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 9530027K12Rik, D3Ertd330e, far upstream element (FUSE) binding protein 1, far upstream element (FUSE) binding protein 4, far upstream element-binding protein 1, FBP, Fubp, Fubp1, Fubp4, FUSE-binding protein 1
Official Symbol: Fubp1
Accession Number(s): NP_476513.2
Human GeneID(s): 8880
Non-Human GeneID(s): 51886 (mouse), 654496 (rat)

Immunogen

Peptide with sequence C-DQIVEKGRPAPGFHH, from the internal region of the protein sequence according to NP_476513.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4kDa according to NP_476513.2). Recommended concentration: 0.03-0.1µg/ml.
IHC: In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration: 4-8µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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