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Goat Anti-E2F1 (Drosophila, aa480-493) Antibody (EB11712)

Related Products: EBP11712 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11712 Goat Anti-E2F1 (Drosophila, aa480-493) Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Drosophila $411.00 Elite

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EB11712 1
EB11712 (0.5ug/ml) staining of the Drosophila Eye-Antennal Imaginal Disc. Data obtained from Dr. Nam-Sung Moon, McGill University, Montreal, Canada.
Click here to download PDF datasheet.

Target Protein

Principal Names: CG6376, CG6376-PA, CG6376-PB, CG6376-PC, dE2F, dE2F1, def21, DmE2F-1, Dmel\CG6376, Dmel_CG6376, Dp, drosE2F1, DRTF1/E2F, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2f, E2F transcription factor, E2F1, E2F-1, E2f-PA, E2f-PB, E2f-PC, E2-promoter binding factor, Evar(3)164, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729
Official Symbol: E2F1
Accession Number(s): NP_524437.2
Important Comments: Reported isoforms represent identical protein: NP_524437.2; NP_732646.1; NP_732647.1

Immunogen

Peptide with sequence C-TKGLFQTPYRSARN, from the internal region of the protein sequence according to NP_524437.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence:This antibody shows identical pattern in the Drosophila eye-antennal imaginal disc as was observed before with a different antibody (Moon et al, Mol Cell Biol. 2006 Oct;26(20):7601-15. PMID: 16954388). Recommended concentration: 0.3-1ug/ml

Species Reactivity

Tested: Drosophila
Expected from sequence similarity: Drosophila

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