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Code Name Applications Availability Size Tested species Price Grade  
EB11760 Goat Anti-CTGF Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Waltham, MA 02452
Ph. (800) 832-2611
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EB11760 1
EB11760 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11760 2
EB11760 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11760 3
EB11760 (6µg/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11760 4
EB11760 Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CCN family member 2, CCN2, connective tissue growth factor, CTGF, HCS24, hypertrophic chondrocyte-specific protein 24, IBP-8, IGF-binding protein 8, IGFBP8, IGFBP-8, insulin-like growth factor-binding protein 8, MGC102839, OTTHUMP00000017213, NOV2
Official Symbol: CCN2
Accession Number(s): NP_001892.1
Human GeneID(s): 1490
Non-Human GeneID(s): 14219 (mouse), 64032 (rat)
Important Comments: Immunizing peptide overlaps disulphide bond

Immunogen

Peptide with sequence TLPVEFKCPDGE, from the internal region (near C Terminus) of the protein sequence according to NP_001892.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Rat Liver. Recommended concentration: 6-8µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membrane of NIH3T3 and HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in IF on Mouse:
Felipe Paredes, Holly C Williams, Izabela Suster, Macarena Tejos, Roberto Fuentealba, Bethany Bogan, Claire M Holden, Alejandra San Martin
Metabolic regulation of the proteasome under hypoxia by Poldip2 controls fibrotic signaling in vascular smooth muscle cells.
Free Radic Biol Med. 2023 Feb 1:195:283-297.
PMID: 36596387

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