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Goat Anti-EEF1A2 Antibody (EB11854)

Related Products: EBP11854 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11854 Goat Anti-EEF1A2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human.Mouse, Rat $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11854 1
EB11854 (0.5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11854 2
EB11854 (0.5µg/ml) staining of Mouse (A+C) and Rat (B+D) Skeletal Muscle (A+B) and Heart (C+D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: EEF1A2, eEF1A-2, EEF1AL, EF1A, EF-1-alpha-2, elongation factor 1-alpha 2, elongation factor-1 alpha, eukaryotic elongation factor 1 A-2, eukaryotic translation elongation factor 1 alpha 2, FLJ41696, HS1, OTTHUMP00000031776, statin S1, statin-S1, STN, STNL
Official Symbol: EEF1A2
Accession Number(s): NP_001949.1
Human GeneID(s): 1917
Non-Human GeneID(s): 13628 (mouse), 24799 (rat)

Immunogen

Peptide with sequence C-NPATVPFVPIS, from the internal region of the protein sequence according to NP_001949.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 50kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 50.5kDa according to NP_001949.1). The same band is observed in lysates of Mouse and Rat Heart and Skeletal Muscle. Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Human.Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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