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Goat Anti-myeloperoxidase Antibody (EB11953)

Code Name Applications Availability Size Tested species Price Grade  
EB11953 Goat Anti-myeloperoxidase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB11953 1
EB11953 (0.3µg/ml) staining of Human Peripheral Blood Lymphocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MPO, myeloperoxidase
Official Symbol: MPO
Accession Number(s): NP_000241.1
Human GeneID(s): 4353
Important Comments: The immunizing peptide represents the N terminus of the mature protein.

Immunogen

Peptide with sequence C-DKAYKERRESIKQ, from the internal region (near N terminus) of the protein sequence according to NP_000241.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 85kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 83.9kDa according to NP_000241.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human