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Code Name Applications Availability Size Tested species Price Grade  
EB12033 Goat Anti-TNNT3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12033 1
EB12033 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12033 2
EB12033 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TNNT3, troponin T type 3 (skeletal, fast), TNTF, beta TnTF, beta-TnTF, fTnT, fast skeletal muscle troponin T, troponin T, fast skeletal muscle, troponin-T3, skeletal, fast
Official Symbol: TNNT3
Accession Number(s): NP_006748.1; NP_001036246.1; NP_001036247.1
Human GeneID(s): 7140
Non-Human GeneID(s): 21957 (mouse), 24838 (rat)
Important Comments: This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). Reported variants represent identical protein: NP_001284575.1, NP_001036247.1

Immunogen

Peptide with sequence C-TTLRSRIDQAQKHSK, from the C Terminus of the protein sequence according to NP_006748.1; NP_001036246.1; NP_001036247.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03µg/ml.
IHC: In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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