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Code Name Applications Availability Size Tested species Price Grade  
EB12036 Goat Anti-TNNI2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc
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EB12036 1
EB12036 (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TNNI2, troponin I type 2 (skeletal, fast), AMCD2B, DA2B, FSSV, fsTnI, fast-twitch skeletal muscle troponin I, troponin I fast twitch 2, troponin I, fast skeletal muscle, troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform, troponin I, skeletal, fast
Official Symbol: TNNI2
Accession Number(s): NP_003273.1; NP_001139313.1
Human GeneID(s): 7136
Non-Human GeneID(s): 21953 (mouse), 29389 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.

Immunogen

Peptide with sequence KRNRAITARRQHLKS-C, from the N Terminus of the protein sequence according to NP_003273.1; NP_001139313.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 25kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 21.3kDa according to NP_003273.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse, PMID: 34145356.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Tannaz Norizadeh Abbariki, Zita Gonda, Denise Kemler, Pavel Urbanek, Tabea Wagner, Margarethe Litfn, Zhao‑Qi Wang, Peter Herrlich & Olivier Kassel
The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.
Sci Rep. 2021 Jun 18;11(1):12904.
PMID: 34145356

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