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Goat Anti-CD19 (aa422-433) Antibody (EB12091)

Related Products: EBP12091 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12091 Goat Anti-CD19 (aa422-433) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12091 1
EB12091 (2µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD19, CD19 molecule, B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
Official Symbol: CD19
Accession Number(s): NP_001171569.1; NP_001761.3
Human GeneID(s): 930
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001171569.1; NP_001761.3).

Immunogen

Peptide with sequence C-ENDSNLGQDQLS, from the internal region of the protein sequence according to NP_001171569.1; NP_001761.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kDa according to NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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