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Goat Anti-EEF1E1 / AIMP3 / p18 Antibody (EB12188)

Related Products: EBP12188 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12188 Goat Anti-EEF1E1 / AIMP3 / p18 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Pig $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12188 1
EB12188 (0.1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12188 2
EB12188 (0.3µg/ml) staining of Pig Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12188 3
EB12188 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EEF1E1, eukaryotic translation elongation factor 1 epsilon 1, AIMP3, P18, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, eukaryotic translation elongation factor 1 epsilon-1,
Official Symbol: EEF1E1
Accession Number(s): NP_004271.1; NP_001129122.1
Human GeneID(s): 9521
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004271.1; NP_001129122.1).

Immunogen

Peptide with sequence C-SKGNKYSAQGERQ, from the internal region (near N Terminus) of the protein sequence according to NP_004271.1; NP_001129122.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Pig
Expected from sequence similarity: Human, Dog, Pig, Cow

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