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Goat Anti-CDH13 / H-cadherin Antibody (EB12228)

Related Products: EBP12228 - Immunizing Peptide, EBP12229 - Immunizing Peptide, Goat Anti-CDH13 / H cadherin (aa285-297) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12228 Goat Anti-CDH13 / H-cadherin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12228 1
EB12228 (0.3µg/ml) staining of Human Heart (A), Amygdala (B) and Hippocampus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12228 2
EB12228 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDH13, cadherin 13, H-cadherin (heart), CDHH, P105, H-cadherin, T-cad, T-cadherin, cadherin-13, heart cadherin
Official Symbol: CDH13
Accession Number(s): NP_001248.1; NP_001207417.1; NP_001207418.1
Human GeneID(s): 1012
Important Comments: The immunizing peptide represents the N terminus of the mature protein. This antibody is expected to recognize isoform 1, 2 and 3 (NP_001248.1; NP_001207417.1; NP_001207418.1).

Immunogen

Peptide with sequence C-DSDRPERSKFRLTGK, from the internal region of the protein sequence according to NP_001248.1; NP_001207417.1; NP_001207418.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 110kDa band observed in Human Heart lysates and 85kDa in Human Brain (Amygdala and Hippocampus) lysates (calculated MW of 78.3kDa according to NP_001248.1). The observed molecular weights correspond to the glycosylated forms. Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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