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Goat Anti-CD44 Antibody (EB12255)

Related Products: EBP12255 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12255 Goat Anti-CD44 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB12255 1
EB12255 (0.03µg/ml) staining of U-2 OS lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12255 2
EB12255 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, extracellular matrix receptor III, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing function and Indian blood group system, hyaluronate receptor, phagocytic glycoprotein 1
Official Symbol: CD44
Accession Number(s): NP_000601.3; NP_001001389.1; NP_001001390.1; NP_001001391.1; NP_001001392.1; NP_001189484.1; NP_001189485.1; NP_001189486.1
Human GeneID(s): 960
Important Comments: This antibody is expected to recognize reported isoforms 1, 2, 3, 4, 6, 7, 8 (NP_000601.3; NP_001001389.1; NP_001001390.1; NP_001001391.1; NP_001189484.1; NP_001189485.1; NP_001189486.1).

Immunogen

Peptide with sequence C-NRDGTRYVQKGEYRT, from the internal region of the protein sequence according to NP_000601.3; NP_001001389.1; NP_001001390.1; NP_001001391.1; NP_001001392.1; NP_001189484.1; NP_001189485.1; NP_001189486.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75-82kDa band observed in lysates of cell line U-2 OS (calculated MW of 81.5kDa according to NP_000601.3 and 76.6 according to NP_001001389.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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