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Goat Anti-Transcription factor E2F1 (aa314-327) Antibody (EB12261)

Related Products: EBP12261 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12261 Goat Anti-Transcription factor E2F1 (aa314-327) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12261 1
EB12261 (1µg/ml) staining of HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: E2F1, E2F transcription factor 1, E2F-1, RBAP1, RBBP3, RBP3, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, transcription factor E2F1
Official Symbol: E2F1
Accession Number(s): NP_005216.1
Human GeneID(s): 1869

Immunogen

Peptide with sequence C-QEVTSEEENRATDS, from the internal region of the protein sequence according to NP_005216.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9kDa according to NP_005216.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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