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Goat Anti-S100B Antibody (EB12316)

Related Products: EBP12316 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12316 Goat Anti-S100B Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12316 1
EB12316 (1µg/ml) staining of Human Olfactory Bulb (A) and Cerebellum (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12316 2
EB12316 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: S100B, S100 calcium binding protein B, NEF, S100, S100-B, S100beta, S-100 calcium-binding protein, beta chain, S-100 protein subunit beta, S100 calcium-binding protein, beta (neural), protein S100-B
Official Symbol: S100B
Accession Number(s): NP_006263.1
Human GeneID(s): 6285

Immunogen

Peptide with sequence C-SHFLEEIKEQEVVD, from the internal region of the protein sequence according to NP_006263.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 10kDa band observed in Human Brain (Olfactory Bulb and Cerebellum) lysates (calculated MW of 10.7kDa according to NP_006263.1). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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