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Goat Anti-ATP5A1 Antibody (EB12389)

Related Products: EBP12389 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12389 Goat Anti-ATP5A1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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ARP American Research Products, Inc
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EB12389 1
EB12389 (0.01µg/ml) staining of Human (A), fetal Mouse (B), adult Mouse (C) and adult Rat (D) Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12389 2
EB12389 (0.01µg/ml) staining of Pig Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP5A1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin
Official Symbol: ATP5F1A
Accession Number(s): NP_004037.1; NP_001244263.1; NP_001001935.1
Human GeneID(s): 498
Non-Human GeneID(s): 11946 (mouse), 65262 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_004037.1; NP_001244263.1; NP_001001935.1). Reported variants represent identical protein: NP_001244264.1, NP_001001935.1. Reported variants represent identical protein: NP_004037.1, NP_001001937.1 .

Immunogen

Peptide with sequence C-RVHGLRNVQAEE, from the internal region (near N terminus) of the protein sequence according to NP_004037.1; NP_001244263.1; NP_001001935.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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