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Code Name Applications Availability Size Tested species Price Grade  
EB12461 Goat Anti-ATP5C1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12461 1
EB12461 (0.01µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12461 2
EB12461 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP5C1, ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1, ATP5C, ATP5CL1, ATP synthase gamma chain, mitochondrial, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, mitochondrial ATP synthase, gamma subunit 1
Official Symbol: ATP5C1
Accession Number(s): NP_005165.1; NP_001001973.1
Human GeneID(s): 509
Non-Human GeneID(s): 11949 (mouse), 116550 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1).

Immunogen

Peptide with sequence C-TLTFNRTRQAVITKE, from the C Terminus of the protein sequence according to NP_005165.1; NP_001001973.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 33kDa band observed in Human Heart and in Rat Heart lysates (calculated MW of 32.9kDa according to NP_005165.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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